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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/03/2021 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
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Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
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Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
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Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus Nal: |
Vaccines. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02811naa a2200349 a 4500
001 2130440
005 2024-02-06
008 2020 bl uuuu u00u1 u #d
100 1 $aCUNHA, A. F.
245 $aComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.$h[electronic resource]
260 $c2020
520 $aABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.
650 $aVaccines
650 $aStaphylococcus Aureus
653 $aImmunoproteomics
653 $aIntramammary infection
653 $aSerological proteome analysis
700 1 $aANDRADE, H. M.
700 1 $aSOUZA, F. N.
700 1 $aFIALHO JUNIOR, L. C.
700 1 $aROSA, D. L. S. O.
700 1 $aSANCHEZ, E. M. R.
700 1 $aGIDLUND, M.
700 1 $aGOTO, H.
700 1 $aBRITO, M. A. V. P.
700 1 $aGUIMARÃES, A. S.
700 1 $aLAGE, A. P.
700 1 $aREIS, L. C.
700 1 $aLIBERA, A. M. M. P. D.
700 1 $aHEINEMANN, M. B.
700 1 $aCERQUEIRA, M. M. O. P
773 $tJournal of Dairy Science$gv. 103, n. 5, p. 4588-4605, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
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Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
18/02/2000 |
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Data da última atualização: |
22/05/2023 |
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Autoria: |
CORDEIRO, D. G.; BATISTA, E. M.; AMARAL, E. F. do. |
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Afiliação: |
DIVONZIL GONCALVES CORDEIRO, CPAF-AC; EDYR MARINHO BATISTA, CPAF-AC; EUFRAN FERREIRA DO AMARAL, CPAF-AC. |
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Título: |
Utilização do equipamento penetrômetro de cone para identificação dos níveis de compactação do solo. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Rio Branco, AC: Embrapa CPAF-AC, 1998. |
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Páginas: |
2 p. |
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Série: |
(Embrapa CPAF-AC. Instruções técnicas, 15). |
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ISSN: |
0104-9038 |
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Idioma: |
Português |
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Conteúdo: |
O trabalho nos mostra que uma das maneiras economicas e práticas de se identificar as reais condições do solo quanto a compactação decorrente do sistema de manejo utilizado e com o penetrometro de cone, pois e identificada a profundidade da camada compactada e se optar pelo implemento mais adequado para a solução deste problema. |
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Palavras-Chave: |
Acre; Appropriate technology; Brasil; Compactacao; Compactación del suelo; Mediciones; Penetometer; Penetrômetro; Penetrômetro de cone; Penetrómetros; Rio Branco; Soil compactation. |
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Thesagro: |
Clone; Compactação do Solo; Diagnostico; Equipamento Agrícola; Medição; Solo; Tecnologia Apropriada. |
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Thesaurus NAL: |
equipment; Measurement; penetrometers; soil; soil compaction. |
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Categoria do assunto: |
--
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAF-AC/3651/1/it15.pdf
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Marc: |
LEADER 01575nam a2200457 a 4500
001 1492528
005 2023-05-22
008 1998 bl uuuu u0uu1 u #d
022 $a0104-9038
100 1 $aCORDEIRO, D. G.
245 $aUtilização do equipamento penetrômetro de cone para identificação dos níveis de compactação do solo.
260 $aRio Branco, AC: Embrapa CPAF-AC$c1998
300 $a2 p.
490 $a(Embrapa CPAF-AC. Instruções técnicas, 15).
520 $aO trabalho nos mostra que uma das maneiras economicas e práticas de se identificar as reais condições do solo quanto a compactação decorrente do sistema de manejo utilizado e com o penetrometro de cone, pois e identificada a profundidade da camada compactada e se optar pelo implemento mais adequado para a solução deste problema.
650 $aequipment
650 $aMeasurement
650 $apenetrometers
650 $asoil
650 $asoil compaction
650 $aClone
650 $aCompactação do Solo
650 $aDiagnostico
650 $aEquipamento Agrícola
650 $aMedição
650 $aSolo
650 $aTecnologia Apropriada
653 $aAcre
653 $aAppropriate technology
653 $aBrasil
653 $aCompactacao
653 $aCompactación del suelo
653 $aMediciones
653 $aPenetometer
653 $aPenetrômetro
653 $aPenetrômetro de cone
653 $aPenetrómetros
653 $aRio Branco
653 $aSoil compactation
700 1 $aBATISTA, E. M.
700 1 $aAMARAL, E. F. do
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